Table 2 List of primers used in PCR. See Methods section for details
Name | Sequence (5′ → 3′) | Target | Expected amplicon size (BP) | Reference |
|---|---|---|---|---|
AbSSU-F | GCGGTAATTCCAGCTCCAATA | 18 rDNA gene, A. bombi | 323 | This work |
NeoR | GACAGCTTCCAATCTCTAGTCG | [23] | ||
CbSSU-F | TTAGGGTTCGATTCCGGAGAG | 18 rDNA gene, C. bombi | 477 | This work |
CbSSU-R | CATGCGTGGATCAGGAAAGGA | This work | ||
NbSSU-F | TGAGGTGATTAATTGGAGGGC | 18 rDNA gene, N. bombi | 404 | This work |
NbSSU-R | CTCCTTGTGGTATTCTTCCGT | This work | ||
BT-F | AGGATTAGACGTTGATACACGAGC | Mitochondrial genome, B. terrestris | 447 | This work |
BT-R | GGATAATCTGAGTATCGTCGAG | This work | ||
XA-F | TCCAATAGGAGGAGGAGATC | COI gene, X. augusti | 657 | This work |
XA-R | CGTCGAGGTATTCCTATCAATCC | This work |